Journal: Biosensors
Article Title: Dual-Oriented Targeted Nanostructured SERS Label-Free Immunosensor for Detection, Quantification, and Analysis of Breast Cancer Biomarker Concentrations in Blood Serum
doi: 10.3390/bios15070447
Figure Lengend Snippet: ( A ) UV–Vis spectra showing the stepwise functionalization and bioconjugation of gold GNUs exhibiting an LSPR peak at 675 nm, PEG-functionalized GNUs, ADH-functionalized GNU-PEG with a peak at 220 nm, and mAb-conjugated GNU-PEG-ADH displaying a peak at 230 nm followed by a characteristic peak at 214 nm after the interaction. ( B ) Magnified view of ( A ) between 200 and 300 nm. ( C ) Magnification of ( B ) between 270 and 290 nm, where the mAb shows higher absorbance than HER-II, and the corresponding results for CA 15-3 from ( D – F ). Note the higher absorbance of CA 15-3 than the mAb.
Article Snippet: HER-II and CA15-3 monoclonal antibodies (mAbs) were purchased from SinoBiological (10004-MM03, HER-II/ErB 2 /CD340 Antibody, Mouse Mab, and 12123-MM05, MUC1/Mucin 1/CD227 Antibody, mouse Mab, respectively; Pennsylvania, USA). summarizes the characteristics of CA15-3 and the clinical status of the corresponding BCS samples.
Techniques: